Cross reactivity of digitoxin and spironolactone in two radioimmunoassays for serum digoxin.
نویسندگان
چکیده
We measured the cross reactivity of two medications--digitoxin and spironolactone--in two digoxin radioimmunoassay (liquid and solid-phase) kit procedures. Both tests showed similar average percentages of cross reactivity with digitoxin (7.2 and 8.9% for intravenous, and 11.9 and 10.9% for oral administration), but no cross reactivity with spironolactone or its metabolites after equal intravenous or oral doses.
منابع مشابه
Cross-reactivity of anti-digoxin antibodies with digitoxin depends on tracer structure.
The most common methods for measuring digoxin concentrations in serum are immunoassays. The prerequisite for exact determination of the digoxin value is an antibody that specifically binds digoxin. Because digitoxin differs from digoxin only in the C-12 hydroxy group, it is difficult to obtain anti-digoxin antibodies that do not cross-react with this compound. During the development of a fluore...
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Laboratories performing analyses for serum cardiac glycosides are sometimes faced with the problem of distinguishing between digoxin and digitoxin in a specimen. The antibodies to the cardiac glycosides supplied with radioimmunoassay kits for these drugs have some measurable degree of cross reactivity. Therapeutic levels of digitoxin are approximately ten times greater than those of digoxin, an...
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Laboratories performing analyses for serum cardiac glycosides are sometimes faced with the problem of distinguishing between digoxin and digitoxin in a specimen. The antibodies to the cardiac glycosides supplied with radioimmunoassay kits for these drugs have some measurable degree of cross reactivity. Therapeutic levels of digitoxin are approximately ten times greater than those of digoxin, an...
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Some factors affecting results of digoxin determinations using one commercially available radioimmunoassay kit are described and discussed. Serum of pregnant women, cord blood, amniotic fluid and serum of patients taking spironolactone may show erroneously high digoxin activity due to lack of specificity of the antiserum. Cross-reaction with digitoxin was found to vary substantially with antibo...
متن کاملLack of specificity of current anti-digoxin antibodies, and preparation of a new, specific polyclonal antibody that recognizes the carbohydrate moiety of digoxin.
Current immunoassays for digoxin do not distinguish digoxin from its glycosidic metabolites. We have synthesized a novel digoxin/bovine serum albumin conjugate via reductive ozonolysis of the lactone ring such that the carbohydrate moiety of digoxin remains intact. Antibodies raised against this conjugate show minimal cross reactivity to digoxigenin, bisdigitoxide, monodigitoxide, digoxigenin, ...
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عنوان ژورنال:
- Clinical chemistry
دوره 24 4 شماره
صفحات -
تاریخ انتشار 1978